Proliferation Inhibited in NCI-H440 Cells with DADS

Objective: To investigate the effect and the mechanism of DADS on the growth of human small cell lung cancer NCI-H446 cells in vitro. Methods: NCI-H446 cell lines were cultured in vitro. Cell proliferation inhibition was measured by MTT assay and cell count experiments. Cell morphologic change was observed by HE staining and AO-EB fluorescence staining. Result: MTT assay showed that the ability of the metabolic MTT reduced significantly and it had a strong cytotoxic response after cultured 48 hours by DADS. IC50values located between 20 ~ 40 μg/ml. Cell count experiments showed that NCI-H446 cells average doubling time extended after exposure to different concentration DADS. HE staining showed that cell volumes and nucleus become smaller while cytoplasm became abundant and stain hard 24 hours after H446 cellular exposure to DADS. AO-EB fluorescence staining showed that the cells morphoous and colour had huge changed that dealed with DADS. Conclusion: DADS could markedly inhibit the proliferation of NCI-H446 and the effect depended on the concentration and the time of DADS.