Proliferation Effect in SW480 Cell Line With DADS

Objective To investigate the inhibitory effect of diallyl disulfide(DADS) on human colon cancer cell line SW480 in vitro. Methods The experiment was divided into two groups: treated group with DADS and untreated group.SW480 cells proliferation was measured by MTT assay and colony forming.The morphology of tumor was observed under optical microscope and electron microscope.Cell cycle distribution was analyzed by flow cytometry(FCM).The expression of proliferating cell nuclear antigen(PCNA) was detected by immunohistochemistry. Results MTT showed that DADS significantly inhibited SW480 cells proliferation with a dose and time-dependent manner,and the colony-formation rate of SW480 cells treated by DADS significantly decreased with a dose-dependent manner.In treated group,the cellular atypia and nuclear-cytoplasmic ratio were decreased,cell organs were abundant in the intracytoplasm,which were observed by optical microscope and ultramicroscope.Flow cytometry revealed that DADS could markedly arrest cell cycle in G2/M phase with a dose-dependent manner.The expression of PCNA were observed in the two groups by Immunohistochemistry,but it was weakened markedly in the treated group(P0.05). Conclusion DADS had significant inhibitory effect on proliferation of human colon cancer SW480 cells in vitro and arrested cell cycle in G2/M,and its molecular mechanism is related to downregulating expression of PCNA.