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Study of Ampelopsin on Apoptosis in Human Gastric Cancer Cells

Tao Lin

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Abstract

Objective:To study ampelopsin on apoptosis in human gastric cancer cells(SGC-7901).Method: Cell proliferation inhibition rate was measured by MTT,The situation in different apoptosis phase was evaluated by flow cytometry(Annexin-V/PI double staining).Result: Ampelopsin could significantly promote early apoptosis in SGC-7901 after 48 h.Ampelopsin of 50 and 75 mg · L-1 induced apoptosis of(29.03±6.21)%,and(43.43±1.00)% with a dose-dependent relationship,significantly higher than that in the control group(11.83±4.67)%(P0.05,P0.01,P0.01).Cells in G1 phase was(52.07±4.41)%,(57.07±2.70)%,(58.43±2.66)%,significantly higher than(42.00±3.04)% in control group(P0.05,P0.01,P0.01).Conclusion: Ampelopsin could induce the SGC-7901cell apoptosis and block the cells in G1 phase.

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Objective:To study ampelopsin on apoptosis in human gastric cancer cells(SGC-7901).Method: Cell proliferation inhibition rate was measured by MTT,The situation in different apoptosis phase was evaluated by flow cytometry(Annexin-V/PI double staining).Result: Ampelopsin could significantly promote early apoptosis in SGC-7901 after 48 h.Ampelopsin of 50 and 75 mg · L-1 induced apoptosis of(29.03±6.21)%,and(43.43±1.00)% with a dose-dependent relationship,significantly higher than that in the control group(11.83±4.67)%(P0.05,P0.01,P0.01).Cells in G1 phase was(52.07±4.41)%,(57.07±2.70)%,(58.43±2.66)%,significantly higher than(42.00±3.04)% in control group(P0.05,P0.01,P0.01).Conclusion: Ampelopsin could induce the SGC-7901cell apoptosis and block the cells in G1 phase.

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Available abstract

Objective:To study ampelopsin on apoptosis in human gastric cancer cells(SGC-7901).Method: Cell proliferation inhibition rate was measured by MTT,The situation in different apoptosis phase was evaluated by flow cytometry(Annexin-V/PI double staining).Result: Ampelopsin could significantly promote early apoptosis in SGC-7901 after 48 h.Ampelopsin of 50 and 75 mg · L-1 induced apoptosis of(29.03±6.21)%,and(43.43±1.00)% with a dose-dependent relationship,significantly higher than that in the control group(11.83±4.67)%(P0.05,P0.01,P0.01).Cells in G1 phase was(52.07±4.41)%,(57.07±2.70)%,(58.43±2.66)%,significantly higher than(42.00±3.04)% in control group(P0.05,P0.01,P0.01).Conclusion: Ampelopsin could induce the SGC-7901cell apoptosis and block the cells in G1 phase.

Key concepts: Apoptosis, Annexin, Pi, Flow cytometry, Chemistry, Cancer, Cancer cell, MTT assay

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