Study on Extraction Technology for Flavonoids from Lotus Leaf

[Objective] The study aimed to optimize the technology conditions of extracting flavonoids from lotus leaf.[Method] With air-dried lotus leaf produced from Hefei area in Anhi Province,its flavonoids was exacted by hot extraction with alcohol.With the ratio of solid to liquid,extraction time and temperature and alcohol concn.as the factors that effected the extraction yield,the technology conditions of extracting flavonoids from lotus leaf were screened through the orthogonal test and the reproducibility and stability of the extraction conditions were validated through single factor test.[Result] Based on the range K value of orthogonal test,the effect of each factor on the extraction yield of flavonoids from lotus leaf in order was extraction temperatureratio of solid to liquidalcohol concn.extraction time.The optimum technology conditions of extracting flavonoids from lotus leaf from the view of saving energy were as follows: extraction temperature of 80 ℃,ratio of solid to liquid of 1∶20,alcohol concn.of 50% and extraction time of 3 h.The single factor test showed that the results from the reproducibility and stability test accorded with that from the orthogonal test.[Conclusion] The study provided some refernce basis for the develplment and utilization of the flavonoids from lotus leaf.