Inhibitory Effect of Shen Qi Guben Recipe on Lewis Pulmonary Carcinoma in Mice

【Objective】To investigate the therapeutic effect and mechanism of Shen Qi Guben Recipe(SQGR) on Lewis pulmonary carcinoma in mice.【Methods】Forty-eight C57/BL mice were randomized into SQGR group(0.5g/mouse/d),cytoxan(CTX)group(80mg/kg/d),SQGR+CTX group and model group.The mice were inoculated with 0.2mL(2×10~6?/mL) of Lewis pulmonary carcinoma cell suspension by axillary subcutaneous injection.On 2~(nd) day of inoculation,mice in SQGR group and SQGR+CTX group were given gastric gavage of SQGR decoction and the treatment lasted 10 days;on the fourth day of inoculation,mice in CTX group and SQGR+CTX group were given intraperitoneal injection of CTX and the treatment lasted 3 days.Twenty-four hours after the last administration,the weight of tumor mass was detected to work out the tumor-inhibitory rate.Meanwhile,the weight of spleen and thymus gland,peripheral white blood cell(WBC) count,T lymphocyte subgroup and tumor necrosis factor α(TNF-α) level were examined.【Results】The tumor mass weight was reduced and the tumor-inhibitory rate was 30.05% in SQGR group.The tumor-inhibitory rate was 84.96% in CTX group and 90.02% in SQGR+CTX group.Peripheral WBC count,T lymphocyte subgroup and TNF-α level were increased in SQGR group and SQGR+CTX group(P0.05 or P0.01)as compared with the model group.The weight of spleen and thymus gland was increased only in SQGR group.【Conclusion】SQGR has an inhibitory effect on Lewis pulmonary carcinoma and its mechanism may be related to the increase of immune function.SQGR has a synergistic action in combination with CTX and can counteract the side effects of CTX.