Effect of Gubenyiliu Prescription II on Immune Function of Lewis Lung Carcinoma-bearing Mice

Objective:To study the effect of Gubenyiliu Prescription Ⅱ(Prescription Ⅱ,PⅡ)on immune function of Lewis lung carcinoma-bearing mice(C57BL/6).Methods:Sixty C57BL/6 mice were divided into six groups:control group,CTX chemotherapeutics group(intraperitoneal injection 30mg/kg CTX),middle dose of PⅡ(M)(35g PⅡ·kg-1·d-1),high dose of PⅡ(H)(70g PⅡ·kg-1·d-1),middle dose of PⅡ with CTX(M+CTX)(35g PⅡ·kg-1·d-1 and 30mg/kg CTX),high dose of PⅡ with CTX(H+CTX)(70g PⅡ·kg-1·d-1 and 30mg/kg CTX),each group had ten mice.MTT assay was used for NK cytoactive,cell multiplication;cell proliferation of splenic lymphocyte;neutral red was used for peritoneal macrophage-phagocytosis;blood picture was analsized by globulimeter.Results:NK cytoactive,cell multiplication of splenic lymphocyte and phagocytosis of peritoneal macrophage of M+CTX and H+CTX groups had been improved notably comparing with CTX group,P0.05.HGB of both two groups had been enhanced to some extent.The average living days of M and H groups were all over 25d,longer than that of control.Conclusion:Prescription Ⅱ can elevate immune function of Lewis lung carcinoma-bearing mice,improve anemia,and prolong living days of carcinoma-bearing mice.