Effect of Allicin on Growth and Apoptosis of Mouse Ascites Melanoma Cell B16-F1

Objective To study the effect of allicin on growth and apoptosis of mouse ascites melanoma cell line B16-F1.Methods After exposed to different concentration of allicin for different time,the survival rate of B16-F1 cells was explored by MTT.Terminal deoxylnucleotide transferase directed X-UTP nick and end labelling(TUNEL) was used to observe apoptosis.RT-PCR was used to study the expression of ras gene.Results Compared with that of the experimental control group,allicin showed significant inhibiting effect on the growth B16-F1 cells and the expression of ras gene at the dosage of 3-15 μg/ml.From 3μg/ml to 6μg/ml concentration of allicin,the apoptotic rate of cells was significantly increased,the expression of ras gene was significantly decreased.Conclusion Allicin can inhibit the growth of mouse ascites melanoma cell line B16-F1 and induce the apoptosis.The expression of ras gene was decreased by allicin.