Extraction of Thiosulfinates in Garlic
XU Shi-ying
Abstract
XU Shi-ying
Abstract
Thiosulfinates are the best known biological active compound in garlic. It is produced by the interaction of nonprotein amino acid alliin with enzyme alliinase (alliin lyase, EC 4.4.1.4). In this paper, thiosulfinates were extracted by solvent under mild condition. The effect of different factors of enzyme hydrolysis and ethanol extract processing on extract ratio of thiosulfinates was studied. Response surface analysis software was used to determine the optimal extration conditions using ethanol as the extractant.
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Thiosulfinates are the best known biological active compound in garlic. It is produced by the interaction of nonprotein amino acid alliin with enzyme alliinase (alliin lyase, EC 4.4.1.4). In this paper, thiosulfinates were extracted by solvent under mild condition. The effect of different factors of enzyme hydrolysis and ethanol extract processing on extract ratio of thiosulfinates was studied. Response surface analysis software was used to determine the optimal extration conditions using ethanol as the extractant.
Key concepts: Alliin, Chemistry, Hydrolysis, Ethanol, Extraction (chemistry), Enzyme, Chromatography, Organic chemistry