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2004•Pharmaceutical Journal of Chinese People's Liberation ArmyRequires access

Determination of Astragaloside IV in Fufang Hanfangji Granule by RP-HPLC

Peng Xue

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Abstract

Aim To develop a method using RP-HPLC for determination the content of astragaloside Ⅳ in Fufang Hanfangji Granule.Methods The Alltech C 18 column(250mm×4.6mm,5μm) was used.The mobile phase was composed of acetonitrile-water (33∶67).The flow rate was 1ml/min.The column temperature was 30℃.An evaporative light scattering detector(ELSD) Model 2000 was used as detector.Results The linear range of astragaloside Ⅳ was 0.976～4.88μg(r=0.999 7,RSD=2.1%).The average recovery rate of astragaloside Ⅳ was 98.10%,and RSD=1.6%(n=5).Conclusion The method is simple and accurate and can be used reliably for the quality control of astragaloside Ⅳ in the compound Hanfangji Granule.

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What this paper is about

Aim To develop a method using RP-HPLC for determination the content of astragaloside Ⅳ in Fufang Hanfangji Granule.Methods The Alltech C 18 column(250mm×4.6mm,5μm) was used.The mobile phase was composed of acetonitrile-water (33∶67).The flow rate was 1ml/min.The column temperature was 30℃.An evaporative light scattering detector(ELSD) Model 2000 was used as detector.Results The linear range of astragaloside Ⅳ was 0.976～4.88μg(r=0.999 7,RSD=2.1%).The average recovery rate of astragaloside Ⅳ was 98.10%,and RSD=1.6%(n=5).Conclusion The method is simple and accurate and can be used reliably for the quality control of astragaloside Ⅳ in the compound Hanfangji Granule.

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Available abstract

Aim To develop a method using RP-HPLC for determination the content of astragaloside Ⅳ in Fufang Hanfangji Granule.Methods The Alltech C 18 column(250mm×4.6mm,5μm) was used.The mobile phase was composed of acetonitrile-water (33∶67).The flow rate was 1ml/min.The column temperature was 30℃.An evaporative light scattering detector(ELSD) Model 2000 was used as detector.Results The linear range of astragaloside Ⅳ was 0.976～4.88μg(r=0.999 7,RSD=2.1%).The average recovery rate of astragaloside Ⅳ was 98.10%,and RSD=1.6%(n=5).Conclusion The method is simple and accurate and can be used reliably for the quality control of astragaloside Ⅳ in the compound Hanfangji Granule.

Key concepts: Astragaloside, Chromatography detector, Chromatography, Granule (geology), Chemistry, Linear range, High-performance liquid chromatography, Content determination

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