2006•Journal of Guangxi Academy of SciencesRequires access

Content Determination of Chlorogenic Acid and Rutin in Different Processed Shanlücha by HPLC

Xie Zhen

Abstract

To select the best processing method for shanlucha and establish a method for content determination of chlorogenic acid and rutin in shanlucha.Using HPLC to determine the contents of chlorogenic acid and rutin in shanlucha.Chlorogenic acid and rutin can be determined by HPLC,the chlorogenic acid showed a good linear relationship in a range of 0.01032～0.6192μg,r=0.9998,the average recovery was 98.86% and RSD was 2.54%,and the rutin also showed a good linear relationship in a range of 0.02004～1.2024,r=0.9999,the average recovery was 102.31% and RSD was 2.17%.The method is simple,accurate,with strong specificity,which can be used for the quantitative determination and control of shanlucha.

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Key concepts: Rutin, Chlorogenic acid, High-performance liquid chromatography, Chromatography, Chemistry, Linear relationship, Linear range, Detection limit