2009•Guiding Journal of Traditional Chinese Medicine and PharmacyRequires access

Determination on Chlorogenic of GuiLingGao by HPLC

LU Pei-rong

Abstract

Objective:To investigate HPLC method for the determination of chlorogenic acid in GuiLingGao(GLG).Methods:Using acetonitrile-phosphoric acid(13∶87)for mobile phase,327 nm for detection length,the chlorogenic acid of sample was reserved for 11.790.Results:The linear rang was from 0.0440 to 2.8186 μg,and the average recovery was 96.94%,RSD%= 1.44%.Conclusion:The method is simple,rapid and reproducible,so it can be used as the quantitative analysis methods for quality control and production.

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Key concepts: Chlorogenic acid, Chromatography, Chemistry, Phosphoric acid, High-performance liquid chromatography, Acetonitrile, Quantitative analysis (chemistry), Organic chemistry