Induction and Differentiation of Wheat Mature Embryos in Different Genotypes

An effective wheat regeneration system was set up via induction of embryonic callus and mature embryos as the explants.The factors which influenced the callus induction and differentiation of three breeds of wheat(Baimai4411,Kangdongzao-30 and 06-4046) were studied.The results showed that 2,4-D(2 mg·L-1) was more advantageous to the induction of callus differentiation.KT(2 mg·L-1) was useful to improve differentiation and regeneration of Kongdongzao-30 and Baimai4411.KT(0.5 mg·L-1) was advantageous to the mature embryo differentiation and the regeneration of 06-4046.The casein hydrolysis(400 mg·L-1) was more advantageous to the callus induction in different wheat breeds.Therefore,MS medium plus 2,4-D(2 mg·L-1),casein hydrolysis(400 mg·L-1) and some KT could promote the induction and differentia-tion of the calluses of wheat mature embryos.