Separation and Preparation of Flavonoids from Zanthoxylum bungeanum by High-speed Countercurrent Chromatography

Flavonoids from Zanthoxylum bungeanum were separated by high-speed countercurrent chromatography.The separation was performed using a two-phase solvent system composed of chloroform,methanol,n-butanol and water(4:3:0.5:2,V/V) at a flow rate of 2.0 mL/min,and rotation speed and detection wavelength were set at 850 r/min and 254 nm,respectively.Six flavonoids were detected by HPLC.The purities of peaks 1,2,3,4,5 and 6 were 72.7%,67.8%,89.6%,96.5%,94.1% and 91.3%,respectively.Their structures were characterized as foeniculin,quercetin-7-glucoside,rutin and quercetin-3-galactoside by MS and1H-NMR.