Separation and purification of the flavonoid from Cuminum cyminum L by high speed countercurrent chromatography

High-speed countercurrent chromatography(HSCCC) with a two-phase solvent system composed of chloroform-methanol-water(4∶4∶2) was applied to separate and purify two flavonoids from Cuminum cyminum L.The mobile phase was performed at a flow rate of 1.8mL/min,while the apparatus rotated at 850r/min and detected at 254nm.Two kinds of flavones were obtained and yielded 26mg and 24mg respectively.The results showed that the purities of two flavones were more than 90% determined by high performance liquid chromatography(HPLC) which was a simple and rapid method.