Effects of bax protein expression and nerve growth factor on rat brain tissue after intracerebral hemorrhage

Objective:To explore the role of bax protein during nerve injury after intracerebral hemorrhage(ICH)and the protective mechanism of nerve growth factor(NGF).Methods:The rat model of ICH was established by injecting 70 μl of self arterial blood into the brain,and the ICH rats were injected with 5 μl of NGF(NGF group)and 5 μl of normal saline(ICH control group)after ICH,respectively.The expression of bax was detected by immunohistochemical method.Results:In NGF group and ICH control group,the expression of bax protein was found 6 hours after ICH,reached peak at 24 hours,and then decreased,but it was still higher than that in saline control group on day 7.No significant difference in the peak time of bax protein expression was found between NGF group and ICH control group,but the expressions of bax protein at each time point were significantly lower in NGF group than in ICH control group(P0.05).Conclusion:The number of apoptotic cells in perihematoma tissues increases after ICH,which aggravates the nerve injury.NGF could significantly decrease the expression of bax protein and thus alleviate the nerve injury and promote the recovery of nerve function.