The effect of erythropoietin on expression of nerve growth factor in intracerebral hemorrhage

【Objective】 We studied the effect of erythropoietin on neurological function deficit,neuron apoptosis and expression of nerve growth factor in rats after ICH.【Methods】 We produed model of ICH according to method of autoblood injecting caudate nucleus with stereo directionlism.110 male Wistar rats were randomLy divided into four groups,normal group,sham operation group,ICH group,EPO treatment group.The technichs of immunohistochemistry and TUNEL were appied to detect expression of NGF,BDNF and apoptosis cells.We appled t-test and LSD-t test to analysis data.【Results】 Contrast to ICH group,the score of neurological behavior in treatment group rats were decreased significantly at 48~168 hours(P0.05).The positive cells of TUNEL NGF andBDNF in ICH group,EPO group obviously increased after 6h,reached peak 72h and decreased after 120h,the positive cells on different time points significantly decreased compared with that of in sham operation group(P0.01).The positive cells of TUNEL in EPO group on different time points significantly decreased compared with that of in ICH group(P0.01).The NGF and BDNF positive cells in EPO group on different time points significantly increased compared with that of in ICH group(P0.01).【Conclusion】 Apoptosis mechanisms may mediate some of the brain injury after ICH,Erythropoietin can protect neurons from injury by increasing expression of BDNF and NGF.