The Study of the Chitosan Microspheres Encapsulated both 5-Fluorouracil and Succinyl Oleanolic Acid

Objective: To prepare the chitosan microspheres able to encapsulate both 5-fluorouracil and succinyl oleanolic acid. Methods: The ion-crosslinked method w as selected to prepare chitosan microspheres. Both 5-fluorouracil and succinyl oleanolic acid w ere entrapped in the chitosan microsphere. The parameters for preparing the microsphere w ere optimized by single factor experiment and the orthogonal experiments. The particle size distribution,zeta potential and the drug release profiles in buffer solution w ere also investigated. Results: Through single factor and orthogonal experiment optimization,entrapment efficiency of microspheres of fluorouracil by ionic crosslinking reached 53. 9%. The mass ratio of 5-fluorouracil and succinyloleanolic acid to chitosan ratio w as 1 ∶ 10 and 1 ∶ 30,respectively. The microspheres w ere round in shape,smooth surface,uniform particle size distribution,average particle diameter of(563 ± 101)nm. In vitro drug release studies show ed slightly retarded release rate of the microspheres.Conclusion: The ion cross-linked method for preparing chitosan microspheres entrapped both5-fluorouracil and succinyl oleanolic acid w as established stably and feasibly. The microspheres show ed good sustained release characteristics.