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2002•Chinese Journal of Coll BiologyRequires access

THE EFFECT OF HUMAN TELOMERASE ANTISENSE RNA ON LIVER CANCER CELLS

Li Jin

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Abstract

The cDNA of hTR( human telomerase RNA)gene was cloned by the means of RT-PCR and inserted into the reverse transcription virus vector(PLNCX)to construct the antisense expression plasmid of hTR gene. By using lipofectin-mediated DNA transfection, the ob- rained plasmid WPS successfully expressed in human liver cancer cells (SMMC-7721). All data suggest that telomerase antisense RNA could significantly inhibit the telomerase activity and proliferation of cells,prolong cell's doubling time and induce the apotosis of cells.

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What this paper is about

The cDNA of hTR( human telomerase RNA)gene was cloned by the means of RT-PCR and inserted into the reverse transcription virus vector(PLNCX)to construct the antisense expression plasmid of hTR gene. By using lipofectin-mediated DNA transfection, the ob- rained plasmid WPS successfully expressed in human liver cancer cells (SMMC-7721). All data suggest that telomerase antisense RNA could significantly inhibit the telomerase activity and proliferation of cells,prolong cell's doubling time and induce the apotosis of cells.

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Available abstract

The cDNA of hTR( human telomerase RNA)gene was cloned by the means of RT-PCR and inserted into the reverse transcription virus vector(PLNCX)to construct the antisense expression plasmid of hTR gene. By using lipofectin-mediated DNA transfection, the ob- rained plasmid WPS successfully expressed in human liver cancer cells (SMMC-7721). All data suggest that telomerase antisense RNA could significantly inhibit the telomerase activity and proliferation of cells,prolong cell's doubling time and induce the apotosis of cells.

Key concepts: Telomerase, Biology, Telomerase reverse transcriptase, Antisense RNA, Molecular biology, Transfection, Complementary DNA, RNA

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