Experimental study on calculous cholecystitis before and after cholecystolithotomy

Objective To study the pathologic changes of the gallbladder in rabbits with calculous cholecystitis and the causes of gallstone recurrence after choleystolithotomy. Methods Twenty- four rabbits were randomly divided into four groups:(1) normal control group (n=5);(2) operative control group(n=5);(3) stone-implanted(S-I) group (n=6),and (4)stone- removed(S-R) group(n=8).The human cholesterol gallstones were implanted into the rabbit gallbladder in stone-implanted and stone- removed rabbits,The pathologic change of the gallbladder was observed by light microscopy 3 months before and after cholecystolithotomy. Results (1)The wet weight of gallbladder in the S-R group [ (1.1±0.06)g] was significant higher than that in S-I group [(0.515±0.1)g] (P0.05). (2)The thickness of gallbladder in S-R group [(1.956±0.23)mm] was no significant different from that in the S-I group [(1.248±0.85)mm] (P0.05);(3)Sever chronic cholecystitis was 33.3% in S-I group 87.5% and in S-R group(P0.05);(4)Both mucous cell and collagen fibre cell in the gallbladder of stone-removed group were proliferous. Conclusions The structure of diseased gallbladder unable to restore to the normal after cholecystolithotomy is likely a cause of gallstone recurrence.