Macroporous Adsorption Resin for the Purification of Flavonoids from Seabuckthorn Pomace

Considering overall adsorption and desorption capacity, X-5 resin was selected out of 7 types of macroporous resins to purify the crude flavonoids extract from seabuckthorn pomace. The optimum process conditions for purifying the crude flavonoids extract using X-5 resin were determined as follows: sample concentration 2 mg/mL, adsorption duration 2 h, amount of distilled water used for washing the resin 4 BV, and amount of 70% ethanol as desorption solvent 4 BV. Optimized purification gave a maximum recovery of flavonoids of 86.78% and an increase in the purity from 11.6% to 34.1%. X-5 resin was used repeatedly 6 times for flavonoids purification, and both the recovery and purity of flavonoids remained higher.