Separation and purification of total flavones from Semen Descuraniae by macroporous adsorption resin

Objective To establish the purification and separation technology for total flavones from Semen Descuraniae by investigating 6 types of macroporous adsorption resin.Method The adsorption capacity,elution ratio and purify of total flavones were used as evaluating criteria.The UV-VIS spectrophotometric method was used in the determination of total flavones content.Results NKA-Ⅱresin had better property of purification and separation.The optimal technological conditions were: the concentration of the sample of Semen Descuraniae,4 g/ml(amount of crude drug); the maximum adsorption capacity for total flavones,12.92 mg/g;the optimal column ratio of diameter to height,1/12; the flow velocity of adsorption,3ml/min ; the eluting reagent, 80% ethanol(5 times of the volume of the resin);eluting velocity , 3ml/min.Conclusion The technology of separating and purifying total flavones from Semen Descuraniae with NKA-Ⅱwas stable and effective, and can be applied to manufacture.