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2007•jOURNAL OF southwest University for NationalitiesRequires access

HPLC determination of matrine in botanical pesticide formulations

Qi Zhang

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Abstract

A method based on RP-HPLC is developed to determine matrine in aqueous solution of 0.3% kushenjianxiaobojianhuangtong. The mobile phase is 0.05 mol/L Sodium dihydrogen phosphate/acetonitrile/methanol/water (930/50/20/20=V/V/V/W) and the detection wave-length is at 205 nm. The standard curve shows good linearity over the range of 0.376～2.256μg (r=0.99997) . The average recovery is 98.25%, the coefficient of variation technical is 1.01%.

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What this paper is about

A method based on RP-HPLC is developed to determine matrine in aqueous solution of 0.3% kushenjianxiaobojianhuangtong. The mobile phase is 0.05 mol/L Sodium dihydrogen phosphate/acetonitrile/methanol/water (930/50/20/20=V/V/V/W) and the detection wave-length is at 205 nm. The standard curve shows good linearity over the range of 0.376～2.256μg (r=0.99997) . The average recovery is 98.25%, the coefficient of variation technical is 1.01%.

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Available abstract

A method based on RP-HPLC is developed to determine matrine in aqueous solution of 0.3% kushenjianxiaobojianhuangtong. The mobile phase is 0.05 mol/L Sodium dihydrogen phosphate/acetonitrile/methanol/water (930/50/20/20=V/V/V/W) and the detection wave-length is at 205 nm. The standard curve shows good linearity over the range of 0.376～2.256μg (r=0.99997) . The average recovery is 98.25%, the coefficient of variation technical is 1.01%.

Key concepts: Matrine, Chemistry, Aqueous solution, Acetonitrile, High-performance liquid chromatography, Chromatography, Coefficient of variation, Methanol

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