Zhou Huai-jun
Abstract
Ri plasmid was developed and a 585bp fragment of rolC gene was amplified by PCR via primer of rolC gene,and the fragment was cloned into vector pUCm-T.Comparison with previously published nucleotide sequence showed that the homology was 100%.But another clone gene nucleotide sequence showed that the homology was 99% with it.
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Ri plasmid was developed and a 585bp fragment of rolC gene was amplified by PCR via primer of rolC gene,and the fragment was cloned into vector pUCm-T.Comparison with previously published nucleotide sequence showed that the homology was 100%.But another clone gene nucleotide sequence showed that the homology was 99% with it.
Key concepts: Homology (biology), Gene, Plasmid, Nucleic acid sequence, Biology, Genetics, Cloning (programming), Primer (cosmetics)