Chang Li
Abstract
A procedure for rapid extraction of Ri plasmid was developed and a 087 kb fragment of rolC gene was amplified by PCR via primer of rolC gene, and the fragment was cloned into vector pGEM-T The result of sequencing showed that the fragment was exactly rolC gene of TDNA of Ri plasmid
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A procedure for rapid extraction of Ri plasmid was developed and a 087 kb fragment of rolC gene was amplified by PCR via primer of rolC gene, and the fragment was cloned into vector pGEM-T The result of sequencing showed that the fragment was exactly rolC gene of TDNA of Ri plasmid
Key concepts: Plasmid, Molecular biology, Cloning (programming), Primer (cosmetics), Biology, Gene, Molecular cloning, DNA