Study on A Deletion Polymorphism of the Angiotensin Converting Enzyme Gene in Pregnancy Induced Hypertension

Objective:To study on a polymorphism and allele frequency of the angiotensin converting enzyme(ACE) gene in pregnancy induced hypertension.Methods: Polymerase chain reaction(PCR) for detection of ACE gene polymorphism was performed to show the deletion/insertion(D/I) polymorphism in intron 16 of ACE gene.490bp(I) and 190bp(D) PCR products were identified,corresponding to the PCR amplification of the allele with or without the insertion.Results: Derived allele frequencies for insertion and deletion were different between pregnancy induced hypertension patients and control subjects.The frequency of D allele gene and percent of ACE DD genotype were higher in pregnancy induced hypertension(0.574 and 44.5%,respectively) compared with the controls(0.385 and 19.0%)(P0.05).Conclusion: There was an excess of DD genotype and the frequency of D allele gene in pregnancy induced hypertension,indicating that genetic variation in the ACE gene could be involved in the risk of patients and the ACE gene may contribute to the pathogenesis of pregnancy induced hypertension.