Agrobacterium-Mediated Plant Transformation of Soybean

Plant meristem and reproductive organs are often taken as the receptor of exogenous gene in Agrobacterium-Mediated plant Transformation.Agrobacterium can interact with the receptor and complete genetic transformation by vacuum infiltration,floral dip,injection and so on.Then transgenic plants can be detected by the screening of antibiotics and molecular tests.In this study,the gene DREB1C encoding a transcription factor was transformed into the apical and axils growing points of soybean seedling through Agrobacterium-mediated plant genetic transformation.Six positive lines were obtained through PCR assay in the T0 generation transgenic lines.The frequency of transformation is 9.2%.The T1 generation transgenic lines have been tested through PCR,Southern blot and RT-PCR and one shows positive.The results indicate that the DREB1C gene has been integrated into the genome of soybean and has a single copy.The gene transcripts successfully under 200 mmol·L-1 NaCl stress.So this non-tissue culture transformation method is simple and it improves the frequency of transformation efficiently.