Transformation of SPS Gene into Soybean via Agrobacterium-mediated Method

The low efficiency of soybean genetic transformation was contributed by restricted soybean genotypes as receptors,the faultiness of tissue culture system,etc.Through Agrobacterium-mediated transformation of cotyledonary node,the most of genetic plants were chimeras.Consequently,it was difficult to select genetic transformation plants.Another popularly used method-Particle Bombardment,whose technique was hard to operate,easily resulted in gene silence.In this research,SPS gene was transformed into embryonic tips of soybean via Agrobacterium-mediated method.The embryonic tip was primary meristem which originated from primordial meristem,therefore it had strong dividing capability.Regeneration cells were derived from genetic cells,which decreased the possibility of being chimeras.The regenerated plants,which were resistant to Kanamycin,were obtained and examined by PCR and Southern blot.The results indicated that the target gene had been transformed into soybean genome.