Study on transforming hrpZpstagene into soybean

【Objective】 In order to breed transgenic soybean which can resist frogeye leaf spot,the present experiment was conducted to transform the resistant gene hrpZpsta into soybean genome.【Method】 The hrpZpsta gene was transformed into cotyledonary node of recipient variety Jilin 30 via agrobacterium-mediated.Transgenic plants were screened with salt-tolerance gene badh,and detected by PCR,Southern blot,RT-PCR.【Result】 The screening concentration of NaCl was 200 mmol/L in phase of shoots.We got 30 positive transgenic plant lines in T1 generation by PCR detecting,and 45 plant lines in T2 generation,and 284 plant lines in T3 generation,which show that hrpZpsta gene could inherit in transgenic plants.The result of southern blot confirmed that hrpZpsta gene had been integrated into soybean genome,and the integration sites were different.RT-PCR further indicated that hrpZpsta gene had been expressed in soybean recipient.【Conclusion】 We obtained transgenic plant lines of T3 generation,and that hrpZpsta gene could inherit in it.