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Study on quality standard of Guishaoqingxue-Mixture

Hu Jin

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Abstract

Objective To establish the method for quality control of Guishao qingxue-Mixture. Methods Paeoniflorin,ferulic acid,chlorogenic acid and baicalin in Guishaoqingxue-Mixture were identified by TLC,Paeoniflorin was determined by HPLC.The condition of HPLC was Eclipse XDB-C18 column, acetonitrile-water(20:80) as mobile phase;the detection wavelength was 230nm.Results Paeoniflorin, ferulic acid,chlorogenic acid and baicalin can be identified by TLC;The calibration cure was linear in the range of 0.04～0.48μg(r = 0.9994)for paeoniflorin.The average recovery was 97.13%,RSD was 1. 18%(n = 6).Conclusion The method is simple,accurate and suitable for the quality control of Guishao-qingxue -Mixture.

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What this paper is about

Objective To establish the method for quality control of Guishao qingxue-Mixture. Methods Paeoniflorin,ferulic acid,chlorogenic acid and baicalin in Guishaoqingxue-Mixture were identified by TLC,Paeoniflorin was determined by HPLC.The condition of HPLC was Eclipse XDB-C18 column, acetonitrile-water(20:80) as mobile phase;the detection wavelength was 230nm.Results Paeoniflorin, ferulic acid,chlorogenic acid and baicalin can be identified by TLC;The calibration cure was linear in the range of 0.04～0.48μg(r = 0.9994)for paeoniflorin.The average recovery was 97.13%,RSD was 1. 18%(n = 6).Conclusion The method is simple,accurate and suitable for the quality control of Guishao-qingxue -Mixture.

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Available abstract

Objective To establish the method for quality control of Guishao qingxue-Mixture. Methods Paeoniflorin,ferulic acid,chlorogenic acid and baicalin in Guishaoqingxue-Mixture were identified by TLC,Paeoniflorin was determined by HPLC.The condition of HPLC was Eclipse XDB-C18 column, acetonitrile-water(20:80) as mobile phase;the detection wavelength was 230nm.Results Paeoniflorin, ferulic acid,chlorogenic acid and baicalin can be identified by TLC;The calibration cure was linear in the range of 0.04～0.48μg(r = 0.9994)for paeoniflorin.The average recovery was 97.13%,RSD was 1. 18%(n = 6).Conclusion The method is simple,accurate and suitable for the quality control of Guishao-qingxue -Mixture.

Key concepts: Paeoniflorin, Ferulic acid, Medicine, Chromatography, Baicalin, Chlorogenic acid, High-performance liquid chromatography, Calibration curve

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