Enhanced the suppression effect of chronic leukemic cell line K562 treated with etoposide and hyperthermia in vitro

Objective:To investigate the suppression effect and apoptosis of chronic leukemic cell line K562 treated with etoposide and hyperthermia.Methods:The working concentration of etoposide against K562 was determined by MTT assay. Then hyperthermia and chemotherapy were used singly or concurrently and the cell survival rates were obtained at 48h. The suppression effect was evaluated by MTT assay. The apoptosis rates of K562 were determined by flow cytometric analyses.Results:The concentration of etoposide in the experiment was defined as its IC50. The 40℃ and 42℃ hyperthennia for 60 min showed obvious inhibition to K562 at 48h(P0.01),etoposide chemotherapy or hyperthermia both obviously inhibited the growth of K562 at 48h (P0.01). Etoposide chemotherapy combined with hyperthermia showed obvious effect to K562. According to flow cytometric analyses, the treatment of hyperthermia and etoposide alone or concurrently could obviously increase the apoptosis rates of K562(P0.01).Conclusion:Etoposide chemotherapy combined with hyperthermia 60min showed obvious suppression effect to K562 at 48h. The synergism might increase apoptosis rates of K562.