Effect of Decocting Safflower and Angelica Alone or Combined on Content Variation of Active Ingredient

OBJECTIVE To compare the content variation of hydroxysafflor yellow A and ferulic acid between single and mixed decoction of Safflower and Angelica.METHODS A RP-HPLC was performed on a Shim-pack VP-ODS C18 column(4.6 mm×150 mm,5 μm)with a gradient elution system composed of methanol and 0.02 mol·L-1 potassium dihydrogen phosphate solutions(adjusted to pH 3.0 with phosphoric acid).The wavelength of UV detection was set at 230 nm.The flow rate was 1.0 mL·min-1.RESULTS The calibration curve of hydroxysafflor yellow A was linear within the range of 2.5-50 mg·L-1(Y=22 821ρ-4 105.1,r=0.999 9).The mean absolute recovery was 100.1% and RSD was 2.2%.The curve of ferulic acid was linear within the range of 0.5-10 mg·L-1(Y=70 219ρ-2 573.9,r=0.999 9).The mean absolute recovery was 101.4% and RSD was 3.0%.CONCLUSION The content of hydroxysafflor yellow A and ferulic acid in mixed decoction is higher than the single decoction of Safflower and Angelica.The mixed decoction is favorable for the extraction of hydroxysafflor yellow A and ferulic acid.The method is convenient,rapid,accurate and successfully applied to detect hydroxysafflor yellow A and ferulic acid simultaneously.