Study on Content Variation of Active Ingredient in Single and Mixed Decoction of Radix Angelica Sinensis and Flos Carthami by HPLC

Objective To study the changes of the contents of ferulic acid and hydroxysafflor yellow A in the single and mixed decoction of Radix Angelicae Sinensis and Flos Carthami,and to explore the characteristics of Chinese herbs compatibility.Methods A RP-HPLC was performed on a Phenomenex C18 column(4.6 mm×150 mm,5 μm) at 30 ℃.The mobile phase consisted of methanol-0.05 %phosphate solution(25 ∶75 V/V,pH 2.45) at the flow rate of 1.0 mL/min.The automatic sample injector was set at 5 ℃and the ultraviolet detector was operated at 320 nm for ferulic acid and 403 nm for hydroxysafflor yellow A.Results The linearity ranges of ferulic acid was in the range of 0.5 ～10.0 μg/mL(Y=3.8372X+0.0219,r=0.999 8),the average recoveries of adding sample were from 92.68 %to 101.13 %,the RSD(n =6) were less than 3.2 %.The linearity ranges of hydroxysafflor yellow A was in the range of 5.0～100.0 μg/mL(Y=1.3647X-0.3714,r=0.999 9),the average recoveries of adding sample were from 100.17 %to 102.97 %,the RSD(n=6) were less than 2.8 %.The content of ferulic acid in the mixed decoction was 142.64 %higher than that in the single decoction of Radix Angelicae Sinensis,and hydroxysafflor yellow A content was 145.37 %higher than that in the single decoction of Flos Carthami.Conclusion The content of ferulic acid and hydroxysafflor yellow A in mixed decoction is higher than the single decoction of Radix Angelicae Sinensisand and Flos Carthami.The method is simple,fast and accurate.It can be used for the quality control of ferulic acid and hydroxysafflor yellow A simultaneously in extracts of medicinal material.