Optimization of simultaneous extraction of water-soluble polysaccharides and total flavone from Lygodium japonicum by tissue culture

The comprehensive extraction technique of water-soluble polysaccharides and total flavone from tissue cultured Lygodium japonicum was studied.Based on single factor experiments,a central composite design(CCD)involving three variables such as extraction temperature,time and solid/liquid ratio at three levels was employed for establishing a quadratic regression model describing total extraction yield of water-soluble polysaccharides and total flavonoids at different levels of the above variables.The optimal technical conditions of extraction by central composite design and response surface analysis(RSA)were as follows:extraction time 2.1h,extraction temperature 80.6℃,solid/liquid ratio of 1∶ 26.2.Under the optimized conditions,the water-soluble polysaccharide yield was 13.241%,the total flavone yield was 3.211%,and both of the total extraction yield was 16.452%.