Extraction Process Optimization of Antivirus Composition from Flos Lonicerae,Fructus Forsythiae,Fructus Arctii with Orthogonal Test

Objective To optimize the extraction process of antivirus composition in Flos lonicerae,Fructus forsythiae and Fructus arctii.Methods The extraction solvent was determined as 50% ethanol by Anti-virus Test.With the contents of chlorogenic acid in Flos lonicerae,forsythiaside in Fructus forsythiae and arctigenin in Fructus arctii and as well as extracts rate as evaluation indexes,the solvent consumption,extraction temperature,abstraction time and extraction times were optimized by the L9(34) orthogonal design.Results The optimum extraction condition was as follows: 6 folds of 50% ethanol,extraction 3 times at 90℃,and 1h each time.Conclusion This method is easy to operate,has better reproducible and can be used for industrial production.