2004•Hubei nongye kexueRequires access
Sterilized method on tissue culture of Taiwan green jujube
Liming Guo
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Abstract
Effects of sterilization on explants and of growth on callus were studied in the tissue culture of Taiwan green jujube of Zizyphus mauritiana by means of 1/600 carbendazim, 75%alcohol, 0.1% mercuric chloride and Tween-20 in order to establish the sterilization method of the tissue culture. The results were as follows: The explant pollution rate was decreased by spraying 1/600 carbendazim 5 days before the explants were used. 75% alcohol sterilized the explants for 30 seconds, 0.1% mercuric chloride with 3～5 drops of Tween-20 sterilized the explants for 5 minutes, which not only decreased the explant pollution rate, but also promoted the callus growth.