Sterilized Methods on Tissue Culture of Winter Ziziphus Jujuba

Effects of sterilization on explants and growth on callus were studied in the stem tip,lamina,stem segment and leafstalk tissue culture of winter Ziziphus jujuba by means of 70% alcohol,mercuric chloride,sodium hypochlorite and tween-20 in order to establish the sterilization method of the tissue culture.The result indicated that 70% alcohol sterilized the explants for 30 seconds,0.1% mercuric chloride with 3～5 drops of tween-20 sterilized the explants for six minutes,which not only decreased the explants pollution rate,but also promoted the survival rate of the explants.