The influence of captopril on cultured human umbilical vein endothelial cells proliferation under different pressure condition

Objectives To clarify whether ambient pressure of culture media affect proliferation of cultured human umbilical vein endothelial cells, and to observe the protect effects of captopril on proliferation. Methods Cells were cultured by modified Jaffes method. Experiments were carried out using cells from the 4 th through 6 th passages. HUVECs were exposed to either 0 mmHg, 120 mmHg, or 180 mmHg. The same culture condition was divided into three groups: control group, drug 1 group, drug 2 group. The changes of proliferation of HUVECs under different pressure conditions were investigated by MTT method, morphology analysis. Results Compared with 0 mmHg, 120 mmHg can induce proliferation of HUVECs at 16 h, 24 h, 48 h( P 0 05); 180 mmHg stimulate proliferation of HUVECs at 16 h, however, at 24 h and 48 h ( P 0 05), cell number was significantly lower however ( P 0 05). An increase in cell viability were observed in captopril (10 -6 ?10 -5 mol/L) treated HUVECs by a dose dependence ( P 0 1). Conclusions 120 mmHg promotes HUVECs growth; 180 mmHg can stimulate HUVECs proliferation at 16 h , and inhibit its proliferation at 24 h and 48 h; Captopril can improve growth inhibition of cultured HUVECs under 180 mmHg.