Polymorphisms of the Porcine Growth Hormone Gene (pGH) in Tibet Pig

The porcine growth hormone(pGH) gene is one of important candidate for development and growth trait.In the present study,the polymorphism of fragment +159～+734 of pGH gene was measured with the methods of PCR-RFLP by restriction endonuclease Dra I,Apa I and Msp I.The results showed that the Tibet pig has no diversity for PCR-Dra I-RFLP of pGH gene.There are four alleles and five genotypes for PCR-Apa I-RFLP of pGH gene,and the genotype CC(299A/432C) is predominant(0.7857).The Tibet pig population has three genotypes for PCR-Msp I-RFLP of pGH gene,and the predominant genotype CT is(0.5089).The frequencies of allele C and allele T are 0.5580 and 0.4420 respectively.By comparing with other breeds,the Tibet pig has differential genotypes for Apa I,and the distribution of alleles for Msp I is uniform relatively,which may be the difference of Tibet pig with other pig breeds.