Effects of Bushen Tongluo Decoction on OPG /RANKL mRNA Expression of in Osteoporosis Rats

Objective: To investigate the effects of Bushen Tongluo decoction(BTD) on mRNA expression of receptor activator of NF-κΒ lingand(RANKL) / osteoprotegerin system(OPG) in the osteoporosis rats by bilateral ovariotomy and explore the molecular mechanism of treating osteoporosis with BTD.Method: Rat model of the osteoporosis was established by bilateral ovariotomy.The rats were divided into six groups randomly: the sham operated group,model group,Xianling Gubao group(5.0 g.kg-1),Bushen decoction group(5.4 g.kg-1),Tongluo decoction group(0.9 g.kg-1),BTD group(6.3 g.kg-1).After ten weeks treatment.The mRNA expression of OPG and RANKL in L3 bone tissues was analyzed by RT-PCR.Result: BTD group,Bushen decoction group and Tongluo decoction group could reduce RANKL mRNA expression,decrease the ratio of RANKL / OPG compared with model group,and there was significant difference(P 0.01).The effect of BTD was superior to Bushen decoction and Tongluo decoction(P 0.05).Conclusion: The effect and related mechanism of BTD in the treatment of primary osteoporosis may be related to regulation of RANKL mRNA and improve RANKL / OPG ratio,thereby inhibiting osteoclast activity and reducing bone resorption.