Immunophenotype characteristics in multiple myeloma cells and its significance

Objective To investigate the immunophenotype characteristics in multiple myeloma(MM)cells and its significance.Methods Four color multiparameter flow cytometry(MP-FCM)was used to test immunophenotype of 30 MM patients and 15 patients with reactive gammopathy.Results The antigen positive expression rale in myeloma cells was as follows: CD138:100%,CD38;100%,CD56:86.67%,GDI 17:33.33%,CD19:3.33%.The results of patients with reactive gammopathy show that CD38 and CD138 were 100%positive,and CD19 was 93.33%positive,CD56 was 6.67%positive, while GD117 was negative.The expression of GD138,GD56 and GD117 in MM group were higher than the control group (P0.01),expression of CD19 was lower than the control group(P0.01)Conclusion MP-FCM is an important diagnostic method for MM and detect the GD38,CD138,CD19,CD56 and GD117 can he helpful for distinguishing myeloma cells from plasma cells.