Analysis of immunophenotypic characteristics in 116 cases of multiple myeloma

Objective To investigate the immunophenotypic characteristics of 116 cases of multiple myeloma(MM) and the relation between the immunophenotype and clinical features.Methods Immunophenotyping was performed in 116 patients of MM by three color multiparameter flow cytometry analysis using a panel of monoclonal antibodies.Results In 58 cases of MM,all cases expressed CD38,the positive ratios of other antigen such as CD19,CD138,CD56,CD20,CD33,CD117,CD45 were 10.3%,96.6%,60.7%,3.4%,70.0%,25.6%,17.2%,respectively.None of the MM cases expressed CD7,CD34.Lack of CD45 expression was associated with greater frequencies of destruction of bone,higher levels of β2-microglobulin and C reaction protein(CRP).Expression of CD19 was associated with higher frequency of advanced international staging system stages,higher levels of β2-microglobulin and CRP,greater frequencies of anemia.Conclusion The expression of CD45 and CD19 could be important clinical prognosis value for MM,which might be helpful for identifying the prognostic factors and guiding clinical treatment in MM.