To Investigate the Effect of Chinese Herb Recipe on the Activity of ERK in Early Diabetic Nephropathy Rats

Objective:To observe the effect of Chinese Herb recipe on the expression of ERK1/2`lipidic`metabolism`oxygen-derived free radicals` in early diabetic nephropathy rats,try to explore the possible mechanism of ERK1/2 activity and the protection role of Chinese Herb recipe on diabetic nephropathy.Methods:The rats were fed with high sucrose and fat diet in a month to make the diabetic nephropathy model established by intra-abdominal injection of STZ.The experimental rats were randomly divided into normal contrast group,pattern group and treated group.Then the rats were treated with Chinese Herb recipe for six weeks.Blood glucose`ALB TC`TG`LDL`MDA were measured.The level of phospho-ERK1/2 was examined by western blot.The renal tissue of rats were observed with light microscope and electron microscope.Results:The levels of ALB`TC`TG`LDL`MDA in Chinese Herb recipe gronp were significantly lower than that in modern group and SOD increased obviously.The expression of pERK1/2 of renal cortex in treated group were significantly lower than that of in modern group.Chinese Herb recipe can relieve the pathological change of renal tissue.Conclusion:① pERK1/2 was participated in the occurring and developing of diabetic nephropathy.② The activity of pERK1/2 may have relation with the reduce high blood lipids and inhibit oxidative stress..③It may have relation with inhibiting the activity of pERK1/2 reducing blood lipids and oxygen-derived free radicals that Chinese Herb recipe can treat early diabetic nephropathy.