Construction and Identification of the Prokaryotic Expression Plasmid of Human MMP-1 Gene Fragment

Objective To construct the prokaryotic expression vector of human MMP-1 gene fragment. Methods The total RNA was extracted from human liver tissue and used as a template for reverse-transcription. After PCR amplification,a 1432bp fragment was obtained and cloned into T vector. After digested with restricted enzyme,the target fragment was subcloned into plasmid pMAL-c2x,the recombinant plasmid was transferred into JM109. We analyze the fragment with restricted enzyme and nucleotide sequencing. Results We obtained human MMP-1 gene and its prokaryotic expression vector. Conclusion We obtained the prokaryotic expression vector of human MMP-1 gene fragment ,which will further help in making MMP-1 fusion protein and the polyclonal antibody against MMP-1.