Construction of Eukaryotic expression Recombinant Plasmid with Human Matrix Metalloproteinase I Gene

To construct expression recombinant plasmid and to analyze the sequence of human matrix metalloproteinase Ⅰ gene (MMP1). The full human MMP1 cDNA was amplified by reverse transcription polymerase chain reaction (RT PCR). The obtained fragment of 1 407 bp was inserted into pcDNA3 vector and transformed into E.Coli DH5α and positive clone was selected. Also the automatic DNA sequencer sequenced the full fragment. A full human MMP1 fragment was obtained, and a base substitution(C to A) of coding region at the position 1 318 was compared with the GeneBank information. Eukaryotic expression recombinant plasmid with MMP1 fragment was successfully constructed. [Conclusion] The human MMP1 cDNA clone is constructed by RT PCR, and recombinant plasmid pcDNA3 MMP1 is successfully constructed;It may provide a basis for further anti liver fibrosis gene therapy.