Study on Gene Cloning and Prokaryotic Expression of Human Interleukin-2

To construct a recombinant espression vector of human Interleukin-2,and eapress in prokaryotic cells,which is helpful for the further purification of recombinant proteins and antibodies production.Methods:Amplify the h-IL2 gene by PCR,the DNA fragments were cloned into pMD-18T simple vectors,transformed into competent DH5α,recombinant plasmid PCR,restriction enzyme digest and sequencing confirmed that the fragment was h-IL2 and the sequence was identical to that published in GenBank.(NM_000586.3).Then the h-IL2 gene were cloned into pGEX-4T-1 constructing expression vectors,transformed into competent BL21(DE3),induced by IPTG,SDS-PAGE verification successful expression of the fusion protein GST-IL2.