The effect of TNF-α expression on ventricular arrhythmias in rats after myocardial infarction

Objective:To investigate the effect of TNF-α expression on ventricular arrhythmias in rats with myocardial infarction.Method:Sixty male Wistar rats were randomly divided into three groups:Sham group (n=20),MI group (n=20) and rhTNFR:Fc group (n=20).Rats with MI were established by liquid nitrogen cryoinjury method.Programmed electrical stimulation was imposed on the hearts to induce ventricular arrhythmia 30 days after myocardial infarction.Protein expression level of TNF-α was detected by western blot and laser scanning confocal 30 days after myocardial infarction.Result:Compared with Sham group,the protein expression levels of TNF-α were significantly increased and the incidence of ventricular arrhythmias was significantly higher in MI group (P0.05).Compared with MI group,the protein expression levels of TNF-α expression were significantly reduced and the incidence of ventricular arrhythmias also significantly lower in rhTNFR:Fc group (P0.05).Conclusion:There is relationship between TNF-α expression and ventricular arrhythmias in rats with MI.