Effect of IL-17 expression on ventricular arrhythmias in post-myocardial infarction rats

Objective:To investigate the effect of IL-17 expression on ventricular arrhythmias in rats with myocardial infarction(MI). Method:Liquid nitrogen cryoinjury method was used in rats to establish MI model.The MI model rats were randomly divided into 3 groups,MI group,IgG group and IL-17 antibady group.Then each group was randomly divided into 3 sub-groups: 2 week,4 week and 8 week group.Sham group was established.The first day after operation,PBS solution were intraperitoneally injected to sham group and MI group,mouse isotype IgG1 and PBS to IgG group,monoclonal human IL-17 antibody and PBS to IL-17 antibady group,5 times every 2 days.Programmed electrical stimulus was imposed on the rat hearts to induce ventricular arrhythmia(ventricular tachycardia and ventricular fibrillation).The evoked electrocardiograms and evoked rates were recorded.The protein expression of IL-17 was examined by Western blot and LSCM. Result:Compared to sham group,expression of IL-17 in myocardium in MI group,IgG group and IL-17 antibody group increased obviously(P0.05) at 2 weeks、4 weeks and 8 weeks after operation.The evoked rates of ventricular arrhythmia also increased(P0.05).Compared to MI group and IgG group,expression of IL-17 in IL-17 group was lower(P0.05),and the evoked rates decreased too(P0.05).While,obvious differences were not found between MI group and IgG group.Statistic analysis showed that there was a positive linear correlation between expression of IL-17 and theevoked rate(r=+0.88,P0.05). Conclusion:IL-17 expression significantly increased after MI.There is a relationship between a large number of IL-17 expression and ventricular arrhythmias in rats with MI.High expression of IL-17 may induce or promote the formation of the reentrant circuit of ventricular arrhythmias in post-myocardial infarction rats.