Analysis of Organic Acids in Fermented Capsicum by Reverse Phase High Performance Liquid Chromatography

A method for simultaneous determination of 8 organic acids in fermented Capsicum by reverse phase high performance liquid chromatography was developed. Hypersil-ODS2 column(4.6 mm×200 mm, 5μm)was used at 17℃ and mobile phase was 2% CH3OH-0.05 mol/L KH2PO4(pH 2.8)with flow rate of 0.8 ml/min. Detection wavelength was 214 nm. The relative standard deviations were lower than 3%, and the recoveries were 92.87% to 106.15%. In this study, two methods (extracted with heating or ultrasonic) were used to extract organic acids, it is proved that extraction with heating is better than ultrasonic.