Protective effect of rice bran antioxidant peptides on liver mitochondria in aging mice induced by D-galactose

Objective:To observe the protective effect of rice bran antioxidant peptides on liver mitochondria in aging mice induced by D-galactose(D-gal),and explore the possible mechanism preliminarily.Method:Sixty Kunming mice were randomly divided into five groups,namely young control group,aging model group and three rice bran peptides-treated groups.The aging model mice were daily given subcutaneous injection of D-gal on the nape back(100mg/kg·bw).Besides,mice in three rice bran peptides-treated groups were daily perfuse respectively with the dose of 100,200 and 500mg/kg·bw of rice bran antioxidant peptides for 6 weeks continuously,and those in the young control group were daily given subcutaneous injection of saline of the same volume for 6 weeks continuously.The activities of manganese-superoxide dimutase(Mn- SOD),glutathione peroxidase(GSH- Px), catalase(CAT),total anti - oxidation capacity(T- AOC),and the content of malonadehide(MDA) in liver mitochondria of all mice were measured by the detection kits.Result:Compared with aging model group,rice bran peptides could markedly enhance the activities of Mn-SOD,GSH-Px,CAT and T-AOC,and significantly inhibit the rise of MDA content(P0.05 or P0.01),there was a relationship of dose - effect.Conclusion:Rice bran antioxidant peptides can significantly improve the anti-oxidation function and inhibit liver mitochondria damage in aging mice induced by D-gal.The mechanism is probably mediated with the scavenging activity of the peptides on free radical and the increase of the activities of antioxidative enzymes,so lipid peroxidation is prevented.