AN ANALYSIS OF HUMAN PAPILLOMA VIRUS INFECTION IN CERVICAL SPECIMEN

Objective To detect the human papilloma virus(HPV) infection in specimens of cervix in gynecology patients,explore the carcinogenicity of different genotypes of HPV infection,and provide experimental evidence for early diagnosis of cervical cancer. Methods Firstly,the thin-cytologic test(TCT) was used to screen various specimens of cervix,for doubtful specimens,such as chronic cervicitis(CC),cervical intraepthelial neoplasia(CIN) and cervical cancer,a second-generation hybrid capture 2(HC Ⅱ) for HPV-DNA detection and high-risk analysis was carried out. Results A total of 460 samples were detected by TCT,which included CC(303 cases),CIN Ⅰ(30),CINⅡ(45),CIN Ⅲ(47) and cervical cancer(35).Of 460 cases detected,211 were infected with HPV before therapy,the infection rate being 45.87%.The HPV-DNA positive rates were 27.06%,77.87% and 97.14%,respectively,in CC,CIN and cancer;the differences of positive rates of HPV-DNA among CC,CIN and cancer were statistically significant(χ2=79.396,P0.01).While positive rates of HPV-DNA among CINⅠ,CINⅡ and CINⅢ were not statistically different(χ2=1.21,P0.05).The commonly seen genotypes of HPV infection in the cervix were HPV-16,52,58,68,18,39,etc.HPV-DNA content in CIN and cancer was higher than that of CC,the difference was significant(t=5.581,P0.05);and that in cervical cancer was higher than that in CIN(t=7.653,P0.05).HPV-DNA value in cervical cancer was significantly higher than CIN(t=6.624,P0.05).The HPV-DNA levels in CINⅠ-Ⅲ showed an increase by degrees but with no significant differences among themt(r=0.991,P0.05). Conclusion HPV infection plays an important effect on the pathological changes of cervix,for those with abnormal colpocytology findings and high-risk women,a further detection of HPV-DNA is of great significance in early discovery of CIN and cervical cancer.